Cat interleukin 6 (IL-6) ELISA kit (6.25-400 pg/mL)

Cat. No.: OB0125WXX-044

Assay Type: Quantitative sandwich ELISA

Target Species: Cat

Assay Target: IL-6

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Cat interleukin 6 (IL-6) ELISA kit (6.25-400 pg/mL)

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Product Overview

Description: Cat interleukin 6 (IL-6) ELISA kit (6.25-400 pg/mL) is a ready-to-use microwell, strip plate ELISA kit for analyzing the presence of cat IL-6 in the range of 6.25-400 pg/mL.

Assay Principle: This assay employs a two-site sandwich ELISA to quantitate IL-6 in cat serum, plasma. An antibody specific for IL-6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells, and any IL-6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-6 is added to the wells. After washing, Streptavidin conjugated HRP is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells, and color develops in proportion to the amount of IL-6 bound in the initial step. The color development is stopped, and the intensity of the color is measured.

Background: IL-6 is a cytokine with multiple biological functions. It is a potent inducer of the acute phase response, acting on T cells, B cells, hematopoietic progenitors, hepatocytes, and cells of the central nervous system. It plays an important role in the eventual differentiation of B cells into Ig-secreting cells. It also acts as an actin, which is excreted into the bloodstream after muscle contraction and can promote lipolysis and improve insulin resistance.

Synonyms: HGF; HSF; BSF2; IL6; IFNB2; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2

Applications: Cat interleukin 6 (IL-6) ELISA kit (6.25-400 pg/mL) is used to detect the level of cat IL-6 in the serum, plasma, and other biological fluid samples.

Research Area: Inflammatory research; Cytokine research; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 6.25-400 pg/mL

Sensitivity: 2.1 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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