Mouse lipoprotein lipase (LPL) ELISA kit (0.312-20 ng/mL)

Cat. No.: 0126WXX-954

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: LPL

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Mouse lipoprotein lipase (LPL) ELISA kit (0.312-20 ng/mL)

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Product Overview

Description: Mouse lipoprotein lipase (LPL) ELISA kit (0.312-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LPL in mice samples with a range of 0.312-20 ng/mL and a minimum detectable dose (sensitivity) of <0.06 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This experiment utilizes a typical double-sandwich ELISA technique. The microplate is pre-coated with a mouse LDH monoclonal antibody, and the detection antibody is a biotin-labeled polyclonal antibody. Samples and the biotinylated antibody are added to the wells and washed with PBS or TBS. Next, avidin-peroxidase conjugates are added. After washing out the reactants thoroughly, the TMB substrate is used for coloration. The color depth and the concentration of the target factors in the samples are positively correlated.

Background: LPL is an enzyme belonging to the lipoprotein lipase family. Its primary function is to break down triglycerides (a type of fat) in the blood. Specifically, LPL hydrolyzes triglycerides present in circulating lipoproteins such as chylomicrons and very low-density lipoproteins (VLDL). The free fatty acids and monoglycerides released during this process can be utilized by cells to generate energy or stored for later use. LPL's direct relevance to obesity research lies in its pivotal role in fat storage and metabolism. Given its central function in lipid metabolism, LPL has emerged as a key biological factor in obesity studies.

Synonyms: Clearing factor; Clearing factor lipase; EC 3.1.1.34

EC NO.: 3.1.1.34

Applications: Mouse lipoprotein lipase (LPL) ELISA kit (0.312-20 ng/mL) is used to quantify LPL in mouse serum, plasma, cell culture supernatant, and organizations of mouse, providing data to support research in a wide range of areas, including signal transduction, metabolism, obesity, etc.

Research Area: Signal Transduction; Metabolism; Obesity

Specification

Sample Type: Mouse Serum; Plasma; Cell culture supernatant; Organizations

Detection Range: 0.312-20 ng/mL

Sensitivity: <0.06 ng/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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