Mouse carnitine O-palmitoyltransferase 1, liver isoform (CPT1A) ELISA kit

Cat. No.: OB0625WXX-421

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: CPT1A

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Mouse carnitine <i>O</i>-palmitoyltransferase 1, liver isoform (CPT1A) ELISA kit

INQUIRY Datasheet MSDS

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Product Overview

Description: Mouse carnitine O-palmitoyltransferase 1, liver isoform (CPT1A) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CPT1A in mouse with a range of 6.25-400 pg/mL and a specificity of 1.56 pg/mL.

Assay Principle: An antibody specific for CPT1A has been pre-coated on the microtiter plate. Standards and samples are pipetted into the wells, and any CPT1A is bound by the immobilized antibody. After removal of unbound material, a biotin-conjugated antibody specific for CPT1A is added to the wells. After washing, avidin-conjugated horseradish peroxidase (HRP) is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color develops in proportion to the amount of CPT1A bound in the first step. The color development is stopped, and the color intensity is measured.

Background: CPT1A, the liver isoform of carnitine O-palmitoyltransferase 1, is the rate-limiting enzyme located in the mitochondrial outer membrane that controls the transport of long-chain fatty acids into mitochondria for beta-oxidation.

Synonyms: CPT1-L; Carnitine O-palmitoyltransferase I, liver isoform

Applications: Mouse carnitine O-palmitoyltransferase 1, liver isoform (CPT1A) ELISA kit is used to quantify CPT1A in serum, plasma, cell lysates, tissue homogenate samples of mouse, providing data to support a wide range of studies.

Research Area: Lipid metabolism; Obesity research

Specification

Sample Type: Serum; Plasma; Cell lysates; Tissue homogenates

Detection Range: 6.25-400 pg/mL

Sensitivity: 1.56 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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