AdipoUpX™ Human UCP1 MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human UCP1 MSCV Particle (Overexpression) is a professionally engineered vector particle providing efficient and stable overexpression of human UCP1. Known as thermogenin, UCP1 is the hallmark of brown fat and a primary target for "browning" research. Its MSCV structure is optimized for hematopoietic stem cells, stem cells, etc., providing a stable platform for investigating energy dissipation in cellular models. This product facilitates the rapid validation of thermogenic mechanisms and is verified by rigorous QC screening for titer, sterility, and mycoplasma.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: UCP1

Full Name: Uncoupling protein 1

Gene Symbol: UCP; SLC25A7

Gene ID: 7350

RefSeq ID-1: NP_068605.1

RefSeq ID-2: NM_021833.5

Summary: UCP1 is a highly specialized mitochondrial anion carrier protein expressed exclusively in Brown Adipose Tissue (BAT). Its unique mechanism creates a "mitochondrial proton leak," which effectively bypasses the conventional ATP synthase pathway. Instead of generating ATP from the proton gradient, UCP1 allows the potential energy to be released directly as heat (nonshivering thermogenesis). This function is critical for maintaining body temperature. Due to its role in increasing energy expenditure, UCP1 is a major focus in research aiming to combat obesity and metabolic syndrome.

AdipoUpX™ Human UCP1 MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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