AdipoUpX™ Human TNF MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human TNF MSCV Particle (Overexpression) is a high-titer, ready-to-use MSCV retroviral particle professionally engineered for the stable overexpression of the human TNF (TNF-alpha) gene. As a master pro-inflammatory cytokine, TNF-alpha is a key driver of obesity-induced insulin resistance. Its MSCV structure is optimized for hematopoietic stem cells, stem cells, etc., providing a stable system for establishing chronic inflammation models. The product is ready-to-use and undergoes rigorous QC screening to ensure experimental safety and consistency.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: TNF

Full Name: Tumor necrosis factor

Gene Symbol: DIF; TNFA; IMD127; TNFSF2; TNLG1F; TNF-alpha

Gene ID: 7124

RefSeq ID-1: NP_000585.2

RefSeq ID-2: NM_000594.3

Summary: TNF encodes a multifunctional proinflammatory cytokine primarily secreted by macrophages. It signals through TNFR1 and TNFR2 to regulate cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF is implicated in autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis, ankylosing spondylitis, tuberculosis, polycystic kidney disease, and cancer. Mutations affect susceptibility to cerebral malaria, septic shock, and Alzheimer’s disease. Knockout studies in mice highlight TNF’s neuroprotective role.

AdipoUpX™ Human TNF MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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