AdipoUpX™ Human SH2B1 MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human SH2B1 MSCV Particle (Overexpression) is a high-titer, ready-to-use MSCV retroviral particle professionally engineered for the efficient, stable expression of the human SH2B1 gene. This adapter protein is critical for enhancing both leptin and insulin signaling sensitivity. The vector structure is optimized for hematopoietic stem cells, stem cells, etc., providing a robust platform for investigating the molecular basis of signaling defects. Every product undergoes comprehensive quality control, including titer and mycoplasma testing, ensuring results are repeatable and safe.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: SH2B1

Full Name: SH2B adaptor protein 1

Gene Symbol: PSM; SH2B

Gene ID: 25970

RefSeq ID-1: NP_001139267.1

RefSeq ID-2: NM_001145795.2

Summary: SH2B1 is an intracellular adaptor protein defined by its SH2 domain, functioning as a vital integrator of signals from growth factor and cytokine receptors, particularly the leptin and insulin receptors. By binding to and modulating the activity of downstream kinases (such as JAK and IRS), SH2B1 effectively controls pathways governing cell growth, proliferation, and metabolism. Genetic polymorphisms within SH2B1 are strongly linked to the human metabolic syndrome cluster: obesity, severe insulin resistance, and hypertension, cementing its status as a critical metabolic checkpoint.

AdipoUpX™ Human SH2B1 MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

Related Products