Product Overview
Description:
AdipoUpX™ Human RAI1 MSCV Particle (Overexpression) is a high-titer MSCV particle designed for the specific and efficient stable expression of the human RAI1 gene. RAI1 is a transcription factor linked to Smith-Magenis Syndrome, characterized by obesity and behavioral changes. This stable system is optimized for stem cells, hematopoietic stem cells, etc., providing a robust platform for investigating the transcriptional control of energy homeostasis. The viral system features a modular design, and all lots are verified through comprehensive quality control screening against contaminants.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
RAI1
Full Name:
Retinoic acid induced 1
Gene Symbol:
SMS; SMCR
Summary:
RAI1 is located in the Smith–Magenis syndrome region on chromosome 17 and is predominantly expressed in neuronal tissues. The protein contains a polymorphic polyglutamine tract and regulates transcription. Altered RAI1 dosage contributes to hyperphagia, abnormal circadian rhythms, and syndromic obesity. Its mouse homolog is induced by retinoic acid, highlighting its role in neurodevelopmental and metabolic processes.
