AdipoUpX™ Human IL10 MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human IL10 MSCV Particle (Overexpression) serves as a robust tool for the stable expression of Interleukin-10, a potent anti-inflammatory cytokine. Overexpressing IL10 helps clarify the mechanisms by which inflammation is mitigated in obese adipose tissue to improve insulin sensitivity. The MSCV vector is engineered for efficient delivery to stem cells, hematopoietic stem cells, and varied mammalian cell lines. All products are screened via thorough quality control—including sterility and titer testing—to ensure experimental safety and high-level gene integration.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: IL10

Full Name: Interleukin 10

Gene Symbol: CSIF; TGIF; GVHDS; IL-10; IL10A

Gene ID: 3586

RefSeq ID-1: NP_000563.1

RefSeq ID-2: NM_000572.3

Summary: IL-10 produces a potent anti-inflammatory cytokine that provides essential immunoregulation. It functions by down-regulating the expression of pro-inflammatory Th1 cytokines and MHC class II antigens on macrophages. Blocking NF-kappa B activity and regulating the JAK-STAT pathway helps maintain metabolic and intestinal immune balance.

AdipoUpX™ Human IL10 MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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