Product Overview
Description:
AdipoUpX™ Human MAGEL2 MSCV Particle (Overexpression) is a powerful gene delivery tool designed for the efficient, stable expression of the human MAGEL2 gene. Loss of this gene is associated with Prader-Willi-like syndrome and severe metabolic dysfunction. Its MSCV backbone is optimized for stem cells, hematopoietic stem cells, etc., providing a robust platform for researching protein recycling and circadian regulation in obesity. Every product undergoes comprehensive quality control, including titer and sterility testing, ensuring results are repeatable and safe.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
MAGEL2
Full Name:
MAGE family member L2
Gene Symbol:
PWLS; nM15; NDNL1; SHFYNG
Summary:
MAGEL2 is an imprinted gene located in the chromosomal region (15q11-q13) associated with Prader-Willi Syndrome (PWS). It is expressed exclusively from the paternal allele. The protein is involved in endosomal protein recycling and axonal transport in neurons. Loss of MAGEL2 expression is a major contributor to the PWS phenotype, which is characterized by life-threatening childhood-onset obesity, hyperphagia (extreme hunger), and reduced energy expenditure. This gene highlights how defects in intracellular protein trafficking can lead to massive weight gain.
