AdipoUpX™ Human ADCY3 MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ADCY3 MSCV Particle (Overexpression) is a high-titer MSCV particle designed for the specific and efficient stable expression of the human ADCY3 gene. This adenylate cyclase is localized to primary cilia and is a major genetic driver of obesity through its role in cAMP signaling. The MSCV vector is optimized for hematopoietic stem cells, stem cells, and other mammalian lineages, ensuring reliable integration for high-fidelity functional validation. The viral system features a modular design for high flexibility and undergoes comprehensive quality control to ensure the highest performance.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: ADCY3

Full Name: Adenylate cyclase 3

Gene Symbol: AC3; AC-III; BMIQ19

Gene ID: 109

RefSeq ID-1: NP_001307542.1

RefSeq ID-2: NM_001320613.1

Summary: ADCY3 is a membrane-associated enzyme that serves as a fundamental signal transducer by catalyzing the conversion of ATP into the pivotal secondary messenger, cyclic AMP (cAMP). This protein is broadly distributed and acts as a central node in numerous cellular processes, including olfaction and neuron signaling. Through the regulation of intracellular cAMP levels, ADCY3 significantly impacts systemic metabolic processes and neuronal function. Genetic variations in ADCY3 have been consistently associated with altered appetite regulation and increased susceptibility to obesity, highlighting its key role in the metabolic network.

AdipoUpX™ Human ADCY3 MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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