Product Overview
Description:
AdipoUpX™ Human PPARGC1B MSCV Particle (Overexpression) is a stable integration tool for the specific and efficient overexpression of the human PPARGC1B (PGC-1β) gene. This transcriptional coactivator regulates mitochondrial biogenesis and lipid metabolism, particularly in brown fat. The vector is optimized for stem cells, hematopoietic stem cells, etc., ensuring robust integration across various mammalian cell types for metabolic research. All viral systems undergo rigorous testing to ensure experimental reproducibility and high-titer performance.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
PPARGC1B
Full Name:
PPARG coactivator 1 beta
Gene Symbol:
PERC; ERRL1; PGC1B; PGC-1(beta)
Summary:
PPARGC1B encodes a transcriptional coactivator that enhances the activity of nuclear receptors and transcription factors such as estrogen receptor alpha, NRF1, and glucocorticoid receptor. It regulates fat oxidation, glucose metabolism, and energy expenditure. Reduced expression is observed in prediabetic and type 2 diabetes patients, and certain variants are associated with increased obesity risk. Three transcript variants encoding different isoforms have been described.
