Product Overview
Description:
AdipoUpX™ Human PNPLA2 MSCV Particle (Overexpression) is a high-titer retroviral particle designed for stable overexpression of the human PNPLA2 gene. PNPLA2 (ATGL) is a key enzyme in triglyceride hydrolysis and lipolysis regulation, central to energy mobilization. The MSCV system ensures stable integration in hematopoietic stem cells, stem cells, and mammalian models. Comprehensive QC ensures experimental reproducibility.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
PNPLA2
Full Name:
Patatin like domain 2, triacylglycerol lipase
Gene Symbol:
ATGL; TTS2; PEDF-R; FP17548; TTS-2.2; iPLA2zeta; 1110001C14Rik
Summary:
PNPLA2 encodes an enzyme responsible for initiating triglyceride hydrolysis in adipose tissue, marking the first step of lipid mobilization from stored fat. It is essential for energy homeostasis, and loss-of-function mutations lead to neutral lipid storage disease with muscle involvement.
