Product Overview
Description:
AdipoUpX™ Human LIPC MSCV Particle (Overexpression) is a high-titer, ready-to-use MSCV retroviral particle professionally engineered for the stable expression of the human LIPC gene. Hepatic lipase (LIPC) is a key enzyme in the metabolism of high-density lipoproteins and triglycerides. Its vector architecture is optimized for stem cells, hematopoietic stem cells, etc., providing a stable platform for investigating lipid flux in obesity models. Every batch is verified through comprehensive quality control (titer, sterility, and mycoplasma detection) to ensure experimental safety and high reproducibility.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
LIPC
Full Name:
Lipase C, hepatic type
Gene Symbol:
HL; HTGL; LIPH; HDLCQ12
Summary:
LIPC encodes Hepatic Lipase, an enzyme synthesized in the liver that tethers to the surface of blood vessels. It plays a dual role as both an enzyme and a ligand, facilitating the hydrolysis of triglycerides and phospholipids within circulating lipoproteins. It is essential for converting intermediate-density lipoproteins (IDL) to low-density lipoproteins (LDL) and for HDL remodeling. LIPC is a key biomarker for obesity and hyperinsulinism; genetic variations in this lipase are frequently linked to familial combined hyperlipidemia and increased risk for Type 2 diabetes.
