AdipoUpX™ Human HNF4A MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human HNF4A MSCV Particle (Overexpression) is a stable integration tool for the specific and efficient overexpression of the human HNF4A gene. This nuclear receptor is a master regulator of hepatic and pancreatic gene expression, crucial for glucose metabolism. The vector is optimized for stem cells and different mammalian cell lines, ensuring reliable integration for studying the genetic basis of MODY. Product quality is ensured by rigorous purification and comprehensive QC screening, including titer and sterility validation.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: HNF4A

Full Name: Hepatocyte nuclear factor 4 alpha

Gene Symbol: TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; TCF-14; HNF4alpha

Gene ID: 3172

RefSeq ID-1: NP_000448.3

RefSeq ID-2: NM_000457.4

Summary: HNF4A encodes a nuclear transcription factor that binds DNA as a homodimer and regulates genes involved in liver, kidney, and intestinal function. It controls the expression of HNF1A and other metabolic genes. Mutations in HNF4A cause monogenic, autosomal dominant non-insulin-dependent diabetes and may influence susceptibility to obesity. Multiple transcript variants generate distinct protein isoforms.

AdipoUpX™ Human HNF4A MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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