Product Overview
Description:
AdipoUpX™ Human ENPP1 MSCV Particle (Overexpression) is a professionally engineered retroviral tool for the robust and stable expression of the human ENPP1 gene. Given its critical role in inhibiting insulin receptor signaling and its association with ectopic calcification, this product is vital for insulin resistance research. The MSCV vector is specifically optimized to maintain high-level expression in hematopoietic stem cells, stem cells, etc., ensuring reliable genomic integration. Every batch is subject to exhaustive quality control—including titer, sterility, and mycoplasma testing—to ensure experimental safety and the highest level of data reproducibility.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
ENPP1
Full Name:
Ectonucleotide pyrophosphatase/phosphodiesterase 1
Gene Symbol:
M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; COLED; PDNP1
Summary:
ENPP1 encodes an enzymatically active glycoprotein localized to the cell membrane as a type II transmembrane protein. Structurally, the functional unit consists of two identical subunits linked by disulfide bonds. ENPP1 exhibits enzymatic activity toward a wide range of nucleotide-based substrates, enabling cleavage of both phosphodiester and pyrophosphate linkages present in nucleotides and nucleotide sugars. Through these reactions, the protein contributes to nucleotide metabolism, including the conversion of nucleoside triphosphates into monophosphates and the degradation of diadenosine polyphosphates. Clinically, pathogenic ENPP1 variants have been reported in disorders involving ectopic calcification and skeletal abnormalities, as well as in metabolic conditions characterized by insulin resistance.
