Product Overview
Description:
AdipoUpX™ Human BBS7 MSCV Particle (Overexpression) is a powerful gene delivery tool designed for the specific and efficient stable expression of the human BBS7 gene. As a core component of the BBSome complex, it is vital for researching primary cilia-mediated nutrient sensing. This stable integration system, optimized for stem cells and various mammalian cell types, allows researchers to study the genetic drivers of syndromic obesity. Clients can flexibly choose report genes through our modular platform, and all viral products pass rigorous quality control validation, including titer and sterility testing.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
BBS7
Full Name:
Bardet-Biedl syndrome 7
Gene Symbol:
BBS2L1
Summary:
BBS7 encodes a foundational structural subunit of the BBSome complex, a molecular machine essential for the formation and function of primary cilia. The BBSome acts as a protein coat that facilitates the trafficking of signaling receptors into the ciliary membrane. While assembly of the complex requires specific chaperonins, the structural integrity provided by BBS7 is vital. Mutations in this gene cause Bardet-Biedl Syndrome (BBS), a multisystem ciliopathy. A hallmark of BBS is early-onset truncal obesity, which occurs because the cilia on neurons cannot properly process appetite-regulating signals.
