Product Overview
Description:
AdipoUpX™ Human BBS10 MSCV Particle (Overexpression) is a professionally engineered vector particle providing efficient and stable overexpression of human BBS10. This chaperonin-like protein is crucial for the assembly of the BBSome complex. The MSCV vector is optimized specifically for hematopoietic stem cells, stem cells, and other mammalian cell lines, ensuring consistent and high-level expression. All products pass through rigorous quality control—including titer and sterility testing—to guarantee experimental safety and reproducibility.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
BBS10
Full Name:
Bardet-Biedl syndrome 10
Gene Symbol:
C12orf58
Summary:
BBS10, another member of the BBS gene family, is associated with Bardet-Biedl syndrome type 10, characterized by progressive retinal degeneration, obesity, polydactyly, renal anomalies, and cognitive impairment. While most BBS proteins localize to cilia, BBS10 is likely a molecular chaperone with distant homology to type II chaperonins, affecting the folding or stability of other ciliary or basal body proteins. Inhibition of BBS10 impairs ciliogenesis in preadipocytes.
