AdipoUpX™ Human AFF4 Ad5 Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human AFF4 Ad5 Adenoviral Particle (Overexpression) is engineered for efficient overexpression of the human AFF4 gene, a scaffold protein essential for transcriptional elongation. This tool serves as a powerful resource for studying the genetic control of adipogenesis and systemic growth. Our platform supports the flexible inclusion of resistance markers, and all particles undergo comprehensive QC for maximum research reliability.

Production Cell Line: HEK293

Viral Backbone: Adenovirus type5 (dE1/E3)

Promoter: CMV

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: All viral preparations have been validated via PCR to confirm the integrity of their vector genomes.

Sterility Test: This product was confirmed to be sterile upon testing.

Mycoplasma Test: This product has passed quality control, confirming that it is free of mycoplasma contamination.

Other QC: We perform customized supplementary testing and in vitro/in vivo transduction assessments based on specific project requirements to analyze the expression levels and biological functions of transgenes.

Storage: Upon receipt of viral preparations, they must be immediately transferred to a -80°C environment for long-term storage.

Stability: The product guarantees excellent stability for 6-12 months when stored continuously at -80°C (and in some cases, up to 2 years). Upon thawing, the working solution is refrigerated at 4°C for up to 2-3 weeks without significant loss of biological activity.

Shipping Condition: Our adenovirus viral products are shipped using dry ice.

Handling Notes: Viral particles are highly sensitive to temperature fluctuations and freeze-thaw cycles. To maintain titer and avoid repeated freeze-thaw cycles, it is recommended to aliquot the vector immediately upon receipt into low-protein-binding tubes and store according to specified conditions. Ensure all operations are performed within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Our company is committed to product quality, and all products have undergone rigorous internal QC inspections. Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application. Users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements throughout all operational processes.

Target Profile

Gene Name: AFF4

Full Name: ALF transcription elongation factor 4

Gene Symbol: MCEF; CHOPS; AF5Q31

Gene ID: 27125

RefSeq ID-1: NP_055238.1

RefSeq ID-2: NM_014423.4

Summary: AFF4 encodes a transcription factor of the AF4 family, best known for its involvement in hematological malignancy. The AFF4 protein acts as an indispensable subunit of the Positive Transcription Elongation Factor b (P-TEFb) complex, which functions to greatly enhance the speed and efficiency of gene transcription. Its translocation is a defining feature of certain acute leukemias; specifically, a chromosomal rearrangement involving AFF4 and the MLL gene on chromosome 11 is a frequent finding in infant acute lymphoblastic leukemia cases involving specific insertions.

AdipoUpX™ Human AFF4 Ad5 Particle (Overexpression)

Product Overview

Specification

Target Profile

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