Recombinant Rat Leptin Protein, Tag-free (E. coli)

Cat. No.: OB0626-WX6

Species: Rat

Origin: Recombinant

Conjugation: Unconjugate

Size:

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Product Overview

Description: Recombinant Rat Leptin Protein is expressed in E. coli as a tag-free protein. Produced in a cost-effective E. coli expression system, without fusion tags, preserving native folding for ligand–receptor interaction and binding studies. Recombinant Rat leptin protein is a highly purified, tag-free metabolic cytokine designed to support rodent-based obesity and diabetes research. It is ideal for stimulating rat primary adipocytes and validating species-specific physiological and neuroendocrine responses without tag interference.

Source: Escherichia coli

Expression Region: Val22-Cys167

Sequence: VPIHKVQDDTKTLIKTIVTRINDISHTQSVSARQRVTGLDFIPGLHPILSLSKMDQTLAVYQQILTSLPSQNVLQIAHDLENLRDLLHLLAFSKSCSLPQTRGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDLSPEC

Molecular Weight: Approximately 16.3 kDa

Bioactivity: The ED₅₀ is ≤1.0 ng/mL as determined by a cell proliferation assay using human Leptin R-transfected BaF3 cells, corresponding to a specific activity of ≥1.0 x 10^6 U/mg.

Tag: Free

Purity: ≥95% by SDS-PAGE

Endotoxin Test: <0.2 EU/μg as determined by the LAL method

Applications: ELISA; WB; Functional Assay; Cell Culture; Binding Assay; SPR; BLI; FACS; Positive Control

Specification

Formulation: Lyophilized from a Tris buffer containing NaCl, formulated with trehalose, mannitol, and Tween-80.

Storage: Storage at -20°C.

Shipping Condition: Shipped with blue ice.

Handling Notes: During shipping, trace amounts of the recombinant protein may adhere to the interior surface of the vial cap. Before opening, it is highly recommended to briefly centrifuge the vial to bring all contents to the bottom. Avoid repeated freeze-thaw cycles to prevent activity loss.

Target Profile

Full Name: Leptin

Alternative Names: rRtLeptin; Obesity factor; Obesity protein; OB; Lep

Gene ID: 25608

Uniprot ID: P50596

Accession Number: NP_037208.1

Summary: Leptin (LEP) is an adipocyte-derived hormone secreted by white adipocytes into the systemic circulation, where it governs energy homeostasis. By binding its receptor in the brain, leptin activates downstream signaling that suppresses feeding and increases energy expenditure, while additionally exerting endocrine actions on immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation, and wound healing. Mutations within LEP or its regulatory regions cause severe and morbid obesity with hypogonadism in patients and contribute to the development and progression of type 2 diabetes mellitus.

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