Recombinant Mouse Leptin Protein, Tag-free (E. coli)

Cat. No.: OB0626-WX8

Species: Mouse

Origin: Recombinant

Conjugation: Unconjugate

Size:

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Product Overview

Description: Recombinant Mouse Leptin Protein is expressed in E. coli as a tag-free protein. Produced in a cost-effective E. coli expression system, without fusion tags, preserving native folding for ligand–receptor interaction and binding studies. Recombinant Mouse leptin protein is a fully bioactive, tag-free cytokine tailored for the most widely used animal models in obesity research. It is optimized for acute or chronic in vivo administration in ob/ob and db/db mice, as well as in vitro 3T3-L1 adipocyte differentiation assays.

Source: Escherichia coli

Expression Region: Val22-Cys167

Sequence: VPIQKVQDDTKTLIKTIVTRINDISHTQSVSAKQRVTGLDFIPGLHPILSLSKMDQTLAVYQQVLTSLPSQNVLQIANDLENLRDLLHLLAFSKSCSLPQTSGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDVSPEC

Molecular Weight: 13-15 kDa

Bioactivity: Measured by its ability to induce proliferation in MCF-7 human breast cancer cells, the ED₅₀ for this effect is ≤2.0 ng/mL. This corresponds to a specific activity of ≥5.0 x 10^5 U/mg.

Tag: Free

Purity: ≥95% by SDS-PAGE

Endotoxin Test: <1.0 EU/μg as determined by the LAL method

Applications: ELISA; WB; Functional Assay; Cell Culture; Binding Assay; SPR; BLI; FACS; Positive Control

Specification

Storage: Storage at -20°C.

Shipping Condition: Shipped with blue ice.

Handling Notes: During shipping, trace amounts of the recombinant protein may adhere to the interior surface of the vial cap. Before opening, it is highly recommended to briefly centrifuge the vial to bring all contents to the bottom. Avoid repeated freeze-thaw cycles to prevent activity loss.

Target Profile

Full Name: Leptin

Alternative Names: Obesity factor; Obesity protein; OB; Lep

Gene ID: 16846

Uniprot ID: Q544U0

Accession Number: NP_032519.1

Summary: Leptin (LEP) is an adipocyte-derived hormone secreted by white adipocytes into the systemic circulation, where it governs energy homeostasis. By binding its receptor in the brain, leptin activates downstream signaling that suppresses feeding and increases energy expenditure, while additionally exerting endocrine actions on immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation, and wound healing. Mutations within LEP or its regulatory regions cause severe and morbid obesity with hypogonadism in patients and contribute to the development and progression of type 2 diabetes mellitus.

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