Recombinant Human Leptin Protein, N-6×His-tag (E. coli)

Cat. No.: OB0626-WX2

Species: Human

Origin: Recombinant

Conjugation: Unconjugate

Size:

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Product Overview

Description: Recombinant Human Leptin Protein is expressed in E. coli with an N-6×His tag. Produced in a cost-effective E. coli expression system, and the His-tag enables straightforward purification and detection. Recombinant human leptin protein-tag is an engineered metabolic cytokine for streamlined detection and purification. It serves as a highly reliable tool for protein-protein interaction analysis (such as SPR/BLI) and as a robust positive control in Western Blot and ELISA development. It is suitable for a range of in vitro and cell-based research applications, including protein characterization, binding, and functional studies.

Source: Escherichia coli

Expression Region: Val22-Cys167

Sequence: VPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLENLRDLLHVLAFSKSCHLPWASGLETLDSLGGVLEASGYSTEVVALSRLQGSLQDMLWQLDLSPGC

Molecular Weight: Approximately 17 kDa

Tag: N-terminal 6×His

Purity: ≥95% by SDS-PAGE

Endotoxin Test: <1.0 EU/μg as determined by the LAL method

Applications: ELISA; WB; Functional Assay; Cell Culture; Binding Assay; SPR; BLI; FACS; Positive Control

Specification

Formulation: Lyophilized from a sterile Tris-HCl buffer containing NaCl and arginine.

Storage: Storage at -20°C.

Shipping Condition: Shipped with blue ice.

Handling Notes: During shipping, trace amounts of the recombinant protein may adhere to the interior surface of the vial cap. Before opening, it is highly recommended to briefly centrifuge the vial to bring all contents to the bottom. Avoid repeated freeze-thaw cycles to prevent activity loss.

Target Profile

Full Name: Leptin

Alternative Names: rHuLeptin; Obesity factor; Obesity protein; OB; Lep

Gene ID: 3952

Uniprot ID: P41159

Accession Number: NP_000221.1

Summary: Leptin (LEP) is an adipocyte-derived hormone secreted by white adipocytes into the systemic circulation, where it governs energy homeostasis. By binding its receptor in the brain, leptin activates downstream signaling that suppresses feeding and increases energy expenditure, while additionally exerting endocrine actions on immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation, and wound healing. Mutations within LEP or its regulatory regions cause severe and morbid obesity with hypogonadism in patients and contribute to the development and progression of type 2 diabetes mellitus.

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