Recombinant Human Leptin Protein, C-Fc-tag (HEK293)

Cat. No.: OB0626-WX4

Species: Human

Origin: Recombinant

Conjugation: Unconjugate

Size:

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Product Overview

Description: Recombinant Human Leptin Protein is expressed in HEK293 cells with a C-Fc tag. Native-like folding in mammalian HEK293 cells preserves proper post-translational modifications such as glycosylation and disulfide-bond formation, while the Fc fusion extends serum half-life and enables protein A/G purification, supporting stable dimeric receptor studies. Recombinant human leptin protein, Fc Tag, is a chimeric fusion protein consisting of human Leptin and an Fc region to mimic the natural homodimeric state. This format significantly extends the protein's half-life, making it ideal for prolonged cell culture treatments requiring high stability.

Source: HEK293 Cells

Expression Region: LEP (Val22-Cys167) + hFc (Glu99-Ala330)

Molecular Weight: 42.2 kDa (Predicted)

Glycosylation: Yes

Tag: C-terminal Fc

Purity: ≥95% by SDS-PAGE

Applications: ELISA; WB; Functional Assay; Cell Culture; Binding Assay; SPR; BLI; FACS; Positive Control

Specification

Formulation: Lyophilized from sterile PBS containing trehalose as a lyoprotectant.

Storage: Storage at -20°C.

Shipping Condition: Shipped with blue ice.

Handling Notes: During shipping, trace amounts of the recombinant protein may adhere to the interior surface of the vial cap. Before opening, it is highly recommended to briefly centrifuge the vial to bring all contents to the bottom. Avoid repeated freeze-thaw cycles to prevent activity loss.

Target Profile

Full Name: Leptin

Alternative Names: rHuLeptin; Obesity factor; Obesity protein; OB; Lep

Gene ID: 3952

Uniprot ID: P41159

Accession Number: NP_000221.1

Summary: Leptin (LEP) is an adipocyte-derived hormone secreted by white adipocytes into the systemic circulation, where it governs energy homeostasis. By binding its receptor in the brain, leptin activates downstream signaling that suppresses feeding and increases energy expenditure, while additionally exerting endocrine actions on immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation, and wound healing. Mutations within LEP or its regulatory regions cause severe and morbid obesity with hypogonadism in patients and contribute to the development and progression of type 2 diabetes mellitus.

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