Recombinant Human Leptin Protein, Biotinylated, N-His-tag (HEK293)

Cat. No.: OB0626-WX3

Species: Human

Origin: Recombinant

Conjugation: Biotinylated

Size:

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Product Overview

Description: Recombinant Human Leptin Protein is expressed in HEK293 cells. The protein features an N-His-tag. Biotin conjugation was performed via standard chemical labeling tag and a targeting the primary amino groups (-NH₂) at both the N-terminus and the lysine (K) residue side chains. tag. Native-like folding in mammalian HEK293 cells preserves proper post-translational modifications such as glycosylation and disulfide-bond formation, and the His-tag enables straightforward purification and detection. Recombinant human leptin protein, biotinylated, is a bioactive cytokine conjugated with biotin to allow ultra-sensitive detection via avidin/streptavidin reagents. It preserves native receptor affinity and is specially designed for flow cytometry, AlphaLISA, and high-throughput screening.

Source: HEK293 Cells

Expression Region: Val22-Cys167

Molecular Weight: 16 kDa

Tag: N-terminal His

Purity: ≥95% by SDS-PAGE

Applications: ELISA; WB; Functional Assay; Cell Culture; Binding Assay; SPR; BLI; FACS; Positive Control

Specification

Formulation: Supplied as a 0.45 µm sterile-filtered liquid solution in PBS.

Storage: Storage at -20°C.

Shipping Condition: Shipped with blue ice.

Handling Notes: During shipping, trace amounts of the recombinant protein may adhere to the interior surface of the vial cap. Before opening, it is highly recommended to briefly centrifuge the vial to bring all contents to the bottom. Avoid repeated freeze-thaw cycles to prevent activity loss.

Target Profile

Full Name: Leptin

Alternative Names: rHuLeptin; Obesity factor; Obesity protein; OB; Lep

Gene ID: 3952

Uniprot ID: P41159

Accession Number: NP_000221.1

Summary: Leptin (LEP) is an adipocyte-derived hormone secreted by white adipocytes into the systemic circulation, where it governs energy homeostasis. By binding its receptor in the brain, leptin activates downstream signaling that suppresses feeding and increases energy expenditure, while additionally exerting endocrine actions on immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation, and wound healing. Mutations within LEP or its regulatory regions cause severe and morbid obesity with hypogonadism in patients and contribute to the development and progression of type 2 diabetes mellitus.

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