Product Overview
Description:
AdipoUpX™ Human LIPE MSCV Particle (Overexpression) is a high-titer MSCV particle designed for the specific and efficient stable expression of the human LIPE (HSL) gene. Hormone-sensitive lipase (HSL) is a key enzyme in the mobilization of stored fats. This stable integration system is optimized for hematopoietic stem cells, stem cells, etc., making it an ideal tool for investigating lipolytic pathways in permanent cellular models. The viral system features a modular design for flexibility and is verified through comprehensive QC screening.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
LIPE
Full Name:
Lipase E, hormone sensitive type
Gene Symbol:
HSL; LHS; REH; AOMS4; FPLD6
Summary:
LIPE encodes hormone-sensitive lipase, which exists in long and short isoforms generated by alternative translational start codons. The long isoform is expressed in steroidogenic tissues, such as the testis, where it hydrolyzes cholesteryl esters for steroid hormone production. The short isoform, expressed in adipose tissue, mobilizes stored triglycerides by converting them into free fatty acids.
