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Arachidonoyl p-nitroaniline
Cat. No.:
OB0626WX-3108
Appearance:
Liquid
Purity:
≥98%
Identity:
Confirmed by TLC and HPLC.
Size:
Add to basket
Product Overview
Description:
Arachidonoyl p-nitroaniline is a synthetic nitroaniline fatty acid amide engineered as a colorimetric kinetic substrate for assessing fatty acid amide hydrolase activity. Leveraging the relatively broad tolerance of this enzyme for diverse non-ethanolamine amide headgroups and truncated or saturated fatty acyl chains compared to nominal endogenous ligands, enzymatic cleavage of this probe releases free p-nitroaniline dyes, enabling high-throughput photometric quantification of enzyme velocity.
Synonyms:
APNA; 5,8,11,14-Eicosatetraenamide, N-(4-nitrophenyl)-, (5Z,8Z,11Z,14Z)-; N-(4-Nitrophenyl)-5Z,8Z,11Z,14Z-eicosatetraenamide; 119520-58-0
CAS No.:
119520-58-0
Compound CID:
35020716
Formula:
C26H36N2O3
Formula Weight:
424.6
Specification
λmax:
204 nm nm; 316 nm
Solubility in DMSO:
50 mg/mL
Solubility in Ethanol:
50 mg/mL
Solubility in DMF:
50 mg/mL
Solubility in Other Solvents:
DMSO:PBS mixture: 1 mg/mL
Storage:
Storage at -20°C.
Applications:
Arachidonoyl p-nitroaniline provides a chromogenic lipid substrate for biochemical studies involving arachidonoyl-containing molecules and enzymatic transformation analysis. It supports substrate characterization, analytical assay development, and investigation of lipid-modifying enzyme activities.
