Comprehensive Monogenic Obesity Panel Test Service
InquiryOverview of Monogenic Obesity
Obesity, a complex disease, arises from an interplay of genetic and environmental influences. While many cases are linked to multiple genes, a notable portion of severe, early-onset obesity, especially when presenting with unusual clinical signs, stems from single-gene defects-known as monogenic obesity. Pinpointing these precise genetic causes is vital for accurate diagnosis, predicting disease progression, and implementing highly tailored, personalized treatment plans that significantly enhance patient outcomes.
Protheragen offers state-of-the-art, comprehensive Obesity Causation Analysis Services, designed to precisely identify the underlying genetic variants responsible for obesity. Leveraging advanced genomic technologies and deep scientific expertise, our service provides researchers with the definitive genetic insights needed to differentiate monogenic obesity from more common forms, enabling a paradigm shift from symptomatic management to precision medicine. This targeted approach is essential for patients who may benefit from specific therapies, dietary interventions, or even bariatric surgery considerations based on their unique genetic profile.
Precision Monogenic Obesity Test for Obesity Management
Protheragen's comprehensive monogenic obesity panel test service follows a meticulously designed workflow to ensure accuracy, efficiency, and the highest quality of results. Each stage is optimized to deliver reliable genetic insights for effective research.
Sample Collection & Submission
The process begins with the secure collection of the biological sample, typically peripheral blood or saliva, which contains genomic DNA. Protheragen provides clear guidelines and collection kits to ensure sample integrity during transit to our state-of-the-art laboratory facilities. Proper sample handling is paramount for subsequent analytical steps.
DNA Extraction & Quality Control
Upon receipt, DNA is meticulously extracted from the submitted sample using validated protocols that maximize yield and purity. Following extraction, rigorous quality control (QC) procedures are performed to assess DNA concentration, integrity, and absence of contaminants. Only samples meeting stringent QC criteria proceed to the next stage, ensuring optimal performance of downstream sequencing.
Targeted Gene Sequencing (NGS)
Our service employs NGS technology, specifically targeting a comprehensive panel of genes known to be associated with monogenic obesity. This highly efficient method allows for the simultaneous sequencing of all relevant exons and splice sites within these genes, providing extensive coverage and high sensitivity for variant detection.
Bioinformatics Analysis
The raw sequencing data generated from NGS undergoes sophisticated bioinformatics analysis. This involves aligning sequence reads to the human reference genome, identifying genetic variants (such as single-nucleotide variants, insertions, and deletions), and filtering out common benign polymorphisms. Our advanced pipelines are designed for robust and accurate variant calling.
Workflow
Applications
- Researchers utilize this comprehensive panel to identify novel genes or pathways involved in obesity, contributing to a deeper understanding of the disease's pathogenesis and the development of new therapeutic targets.
- Genetic testing is used to identify potential syndromic obesity, such as the presence of obesity, developmental delay, or dysmorphological features.
- When conventional dietary and lifestyle interventions fail to achieve significant weight loss, genetic testing uncovers an underlying monogenic cause that may require a different therapeutic strategy.
Advantages
- Comprehensive gene coverage: Our panel includes an extensive list of genes implicated in monogenic obesity, ensuring a broad and thorough test. This breadth of coverage is critical for capturing the diverse genetic landscape of monogenic obesity.
- High accuracy & reliability: Utilizing state-of-the-art NGS technology and rigorous bioinformatics pipelines, Protheragen delivers results with exceptional accuracy and reliability. Every step, from DNA extraction to variant calling, is subject to stringent quality control, ensuring the highest data integrity.
- Faster turnaround times: We understand the urgency of timely tests in settings. Protheragen is committed to providing competitive turnaround times without compromising the quality or accuracy of our results.
Our Solutions for Obesity Research
Publication Data
DOI: 10.3390/jcm11247531
Journal: Journal of Clinical Medicine
Published: 2022
IF: 2.9
Results: The study developed a NGS gene panel, consisting of 72 diagnostic and 244 candidate genes, to predict the outcome of bariatric surgery in severely obese patients. Researchers sequenced 247 adult obese Italian patients and identified deleterious variants in both diagnostic and candidate genes, including mutations linked to Bardet–Biedl syndrome. In a 12-month follow-up of 25 patients, those with deleterious variants showed reduced weight loss after bariatric surgery compared to other patients. The authors suggest that this NGS-based panel could be valuable in evaluating, diagnosing, and managing obese individuals, and in predicting the success of bariatric surgery.
Frequently Asked Questions
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What type of sample is required for the test, and how is it collected?
Typically, a small blood sample is sufficient for our testing. We also process saliva samples in certain cases. Protheragen provides detailed instructions and collection kits to ensure proper sample procurement and secure transportation to our laboratory.
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How long does it take to receive the results after submitting a sample?
We understand the importance of timely results. Our standard turnaround time is competitive, and we strive to deliver comprehensive reports as efficiently as possible without compromising on accuracy. Please reach out to our team for specific turnaround time estimates for your project.
By providing precise genetic insights, Protheragen empowers researchers to unlock the underlying causes of obesity, paving the way for personalized and highly effective interventions. Our commitment to scientific excellence, comprehensive analysis, and actionable reporting ensures that you receive the most reliable information. Please feel free to contact us to discuss how our comprehensive monogenic obesity panel test service benefits your research.
Reference
- Bonetti, G.; et al. Next-generation sequencing of a large gene panel for outcome prediction of bariatric surgery in patients with severe obesity. Journal of Clinical Medicine. 2022, 11(24): 7531. (CC BY 4.0)
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.