Product Overview
Description:
AdipoUpX™ Human PRKCB MSCV Particle (Overexpression) is a high-titer retroviral vector designed for stable overexpression of the human PRKCB gene. PRKCB is a protein kinase involved in insulin signaling and inflammatory pathways, with relevance to metabolic syndrome. The MSCV system enables robust expression in hematopoietic stem cells, stem cells, and other mammalian systems. Comprehensive quality control ensures reproducibility and safety.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
PRKCB
Full Name:
Protein kinase C beta
Gene Symbol:
PKCB; PRKCB1; PRKCB2; PKCI(2); PKCbeta; PKC-beta
Summary:
PRKCB is a member of the protein kinase C family of serine/threonine kinases activated by calcium and diacylglycerol signaling. It phosphorylates a broad range of substrates and is involved in multiple signaling pathways controlling immune activation, apoptosis, endothelial function, and nutrient absorption. In neuronal systems, it has been implicated in stress-related behavioral responses. Multiple isoforms generated through alternative splicing contribute to its functional diversity.
