AdipoUpX™ Human ACOX1 MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ACOX1 MSCV Particle (Overexpression) is an efficient gene delivery system for the stable overexpression of acyl-CoA oxidase 1, the rate-limiting enzyme in peroxisomal fatty acid beta-oxidation. This product is ideal for investigating how peroxisomal lipid metabolism contributes to overall energy homeostasis in obesity. The MSCV vector is optimized for hematopoietic stem cells, stem cells, and other mammalian cell lines, providing flexible reporter gene options. To ensure the highest experimental standards, the particles undergo strict quality control for titer, sterility, and mycoplasma.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: ACOX1

Full Name: Acyl-CoA oxidase 1

Gene Symbol: AOX; ACOX; SCOX; MITCH; PALMCOX

Gene ID: 51

RefSeq ID-1: NP_001171968.1

RefSeq ID-2: NM_001185039.2

Summary: ACOX1 encodes the first enzyme in the peroxisomal fatty acid beta-oxidation pathway. It catalyzes the desaturation of acyl-CoAs, a process that produces hydrogen peroxide while breaking down very-long-chain fatty acids. Genetic defects in ACOX1 lead to the toxic accumulation of these lipids, resulting in severe metabolic and neurological dysfunction.

AdipoUpX™ Human ACOX1 MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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