Product Overview
Description:
AdipoUpX™ Human PRDM16 MSCV Particle (Overexpression) facilitates the stable and efficient expression of PR domain containing 16, a master transcriptional co-regulator that controls the fate of brown and beige adipocytes. By overexpressing PRDM16 in stem cells or mammalian cell lines, researchers can effectively analyze the thermogenic gene program. This ready-to-use MSCV particle provides stable gene integration and undergoes comprehensive quality control, including sterility and titer assays, to guarantee the integrity and reproducibility of every experimental result.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
PRDM16
Full Name:
PR/SET domain 16
Gene Symbol:
MEL1; KMT8F; LVNC8; PFM13; CMD1LL
Summary:
PRDM16 encodes a zinc finger transcription factor that acts as a master switch for adipose tissue identity. It is essential for the development and maintenance of brown fat, which burns energy for thermogenesis. Its ability to convert energy-storing white fat into energy-burning brown fat makes it a primary focus for anti-obesity research.
