Product Overview
Description:
AdipoUpX™ Human NR4A1 MSCV Particle (Overexpression) is a high-titer retroviral vector engineered for stable overexpression of the human NR4A1 gene. NR4A1 is a nuclear receptor involved in metabolic regulation, inflammation control, and energy homeostasis. The MSCV system enables sustained expression in hematopoietic stem cells, stem cells, and other mammalian models, and supports metabolic pathway investigations. All products undergo comprehensive quality control to ensure reproducibility and experimental safety.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
NR4A1
Full Name:
Nuclear receptor subfamily 4 group A member 1
Gene Symbol:
HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
Summary:
NR4A1 encodes an immediate-early nuclear receptor that functions as a transcription factor responsive to cellular stress and mitogenic stimuli. It regulates gene expression programs involved in proliferation, apoptosis, and metabolic adaptation. Under specific conditions, NR4A1 can translocate from the nucleus to mitochondria, where it promotes apoptotic signaling. Multiple isoforms exist due to alternative splicing.
