Product Overview
Description:
AdipoUpX™ Human KLF4 MSCV Particle (Overexpression) is a retroviral tool designed for stable overexpression of the human KLF4 gene. KLF4 is a transcription factor involved in cellular differentiation and metabolic regulation, with reported roles in adipocyte biology and energy balance. The MSCV system ensures durable expression in hematopoietic stem cells, stem cells, and other mammalian cell lines for functional studies. Comprehensive quality control ensures experimental reliability.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
KLF4
Full Name:
Krueppel-like factor 4
Gene Symbol:
EZF; GKLF
Summary:
KLF4 encodes a Kruppel-like zinc finger transcription factor involved in regulating epithelial barrier integrity and cell cycle progression. It contributes to skin barrier formation and is involved in G1/S cell cycle control following DNA damage, partly through interactions with p53 signaling pathways. Loss of KLF4 function in animal models leads to severe postnatal dehydration due to impaired epidermal barrier function. Multiple isoforms are generated through alternative splicing.
