AdipoUpX™ Human ACAT2 MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ACAT2 MSCV Particle (Overexpression) is a high-efficiency retroviral vector designed for stable overexpression of the human ACAT2 gene. ACAT2 encodes acyl-CoA: cholesterol acyltransferase 2, a key enzyme in cholesterol esterification and lipid absorption pathways linked to dyslipidemia and obesity. The MSCV system enables robust, long-term gene expression in hematopoietic stem cells, stem cells, and other mammalian cell lines. Each product undergoes comprehensive quality control, including viral titer determination, sterility testing, and mycoplasma detection, ensuring experimental reproducibility and biosafety.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: ACAT2

Full Name: Acetyl-CoA acetyltransferase 2

Gene ID: 39

RefSeq ID-1: NP_001290182.1

RefSeq ID-2: NM_001303253.1

Summary: ACAT2 encodes cytosolic acetoacetyl-CoA thiolase, an essential enzyme in lipid metabolism. It is involved in the metabolic pathways that handle ketone bodies and fatty acid fragments within the cytoplasm. Interestingly, the gene overlaps with the 3-prime region of the TCP1 gene on the opposite DNA strand, suggesting a complex genomic architecture that may influence its regulation across different species.

AdipoUpX™ Human ACAT2 MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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