LipoKnoxa™ Human SREBF1 shRNA Lentiviral Particle (Silencing)

Sub Cat. No.	TargetSeq	Region	Inquiry
V0126XX515-1	GTGACTTCCCTGGCCTATTTG	CDS	Inquiry
V0126XX515-2	TATTCCGGGAACATCTCTTAG	CDS	Inquiry
V0126XX515-3	TGAGGCTCCTGTGCTACTTTG	3' UTR	Inquiry
V0126XX515-4	Other		Inquiry

Product Overview

Description: LipoKnoxa™ Human SREBF1 shRNA Lentiviral Particle (Silencing) provides a specialized solution for silencing SREBP-1, the master activator of fatty acid synthesis. Developed using optimized U6 and miR30-based shRNA systems, this tool enables researchers to block de novo lipogenesis. Every batch is subjected to total QC verification—covering functional activity, sterility, and mycoplasma—to ensure experimental reproducibility.

Production Cell Line: HEK293T

Promoter: U6; CMV; EF1α; CAG; UBC

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Comprehensive sequencing and PCR analyses were performed to verify the accurate genomic sequence of all viral preparations.

Sterility Test: Confirmed sterile via rigorous microbial analysis; free of bacterial and fungal contamination.

Mycoplasma Test: Rigorous quality control testing has confirmed the absence of mycoplasma contamination in this viral preparation.

Other QC: Customized supplementary testing and in vitro/in vivo assessments are available to verify post-silencing gene expression and biological functionality, ensuring the viral preparations meet the specific potency requirements of your gene interference project.

Storage: Store at -80°C for long-term preservation. Immediate transfer upon delivery is required to prevent loss of viral activity.

Stability: Shelf life: 6–12 months at -80°C (extended stability up to 2 years). Post-thaw: Stable at 4°C for 2–3 weeks without significant degradation of functional titers.

Shipping Condition: Products are delivered on dry ice to maintain the cold chain. Upon arrival, please ensure the presence of dry ice and transfer the vials immediately to -80°C storage.

Handling Notes: Avoid repeated freeze-thaw cycles. Aliquot into low-protein-binding tubes immediately after receipt to maintain optimal activity. For safety and contamination control, all viral handling must be performed inside a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: We ensure product integrity through rigorous QC, but we do not guarantee results in specific research contexts. Proper storage, handling, and total compliance with biosafety laws and safety protocols remain the sole responsibility of the user.

Target Profile

Gene Name: SREBF1

Full Name: Sterol regulatory element binding transcription factor 1

Gene Symbol: HMD; IFAP2; SREBP1; bHLHd1

Gene ID: 6720

RefSeq ID-1: NP_001005291.1

RefSeq ID-2: NM_001005291.3

Summary: SREBF1 acts as a master transcriptional regulator for de novo lipogenesis and sterol biosynthesis. Initially synthesized as an organelle-bound precursor, it moves to the nucleus after proteolytic cleavage to activate genes like the LDL receptor. Its SREBP-1c isoform is specifically induced by insulin, providing a direct molecular link between hyperinsulinemia and the increased hepatic fat synthesis observed in metabolic syndrome.

LipoKnoxa™ Human SREBF1 shRNA Lentiviral Particle (Silencing)

Product Overview

Specification

Target Profile

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