AdipoUpX™ Human PIK3CA Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PIK3CA Lentiviral Particle (Overexpression) is a ready-to-use, high-titer vector particle designed for the efficient and stable overexpression of the human PIK3CA gene. It is a powerful asset for researching the PI3K/AKT signaling axis and its role in nutrient sensing and adipocyte growth. Genome integration ensures the target gene establishes a stable presence in the host cell line. Every batch undergoes comprehensive quality control (titer, sterility, and mycoplasma testing) to ensure experimental reproducibility and safety.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: PIK3CA

Full Name: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha

Gene Symbol: HMH; MCM; CCM4; CWS5; MCAP; PI3K; CLAPO; CLOVE; MCMTC; PI3K-alpha; p110-alpha

Gene ID: 5290

RefSeq ID-1: NP_006209.2

RefSeq ID-2: NM_006218.4

Summary: PIK3CA encodes the catalytic subunit of phosphatidylinositol 3-kinase (PI3K), which phosphorylates phosphoinositides to control intracellular signaling. While frequently associated with cancer, PI3K activity also regulates insulin signaling and energy metabolism, suggesting a potential influence on adiposity. A pseudogene exists on chromosome 22.

AdipoUpX™ Human PIK3CA Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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