AdipoUpX™ Human PLIN1 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PLIN1 Lentiviral Particle (Overexpression) is a high-titer system for the specific and stable expression of the human PLIN1 gene. Perilipin-1 is the primary gatekeeper of adipocyte lipolysis; this stable integration system is an ideal tool for studying lipid droplet stability and hormone-stimulated fat breakdown. All viral particle products undergo comprehensive quality control to ensure safety and efficacy. Clients can freely customize key functional components, including cell-specific promoters, to match their experimental environment.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: PLIN1

Full Name: Perilipin 1

Gene Symbol: PERI; PLIN; FPLD4

Gene ID: 5346

RefSeq ID-1: NP_001138783.1

RefSeq ID-2: NM_001145311.2

Summary: PLIN1 encodes perilipin 1, a protein that coats lipid droplets in adipocytes and regulates their breakdown. When unphosphorylated, it prevents lipolysis, while phosphorylation by cAMP-dependent protein kinase facilitates triglyceride hydrolysis. As the primary substrate for PKA in adipose tissue, PLIN1 plays a critical role in lipid storage and mobilization. Variants or dysregulation of this gene can contribute to abnormal fat accumulation and obesity.

AdipoUpX™ Human PLIN1 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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