LipoKnoxa™ Human IFT27 shRNA Lentiviral Particle (Silencing)

Sub Cat. No.	TargetSeq	Region	Inquiry
V0126XX504-1	TTCGGAAATGCTGGATAAATT	CDS	Inquiry
V0126XX504-2	ACAGTGTGGAACTCTTCATTT	CDS	Inquiry
V0126XX504-3	CCCTGACAACAGGAATGGATT	CDS	Inquiry
V0126XX504-4	Other		Inquiry

Product Overview

Description: LipoKnoxa™ Human IFT27 shRNA Lentiviral Particle (Silencing) targets a protein involved in the intraflagellar transport complex linked to Bardet-Biedl syndrome. Engineered via our dual-strategy platform (U6 and miR30 systems), this tool enables the stable modeling of ciliary-mediated obesity. Our system supports flexible selection of reporter genes and is verified through total QC validation, including functional titer and mycoplasma detection.

Production Cell Line: HEK293T

Promoter: U6; CMV; EF1α; CAG; UBC

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Comprehensive sequencing and PCR analyses were performed to verify the accurate genomic sequence of all viral preparations.

Sterility Test: Confirmed sterile via rigorous microbial analysis; free of bacterial and fungal contamination.

Mycoplasma Test: Rigorous quality control testing has confirmed the absence of mycoplasma contamination in this viral preparation.

Other QC: Customized supplementary testing and in vitro/in vivo assessments are available to verify post-silencing gene expression and biological functionality, ensuring the viral preparations meet the specific potency requirements of your gene interference project.

Storage: Store at -80°C for long-term preservation. Immediate transfer upon delivery is required to prevent loss of viral activity.

Stability: Shelf life: 6–12 months at -80°C (extended stability up to 2 years). Post-thaw: Stable at 4°C for 2–3 weeks without significant degradation of functional titers.

Shipping Condition: Products are delivered on dry ice to maintain the cold chain. Upon arrival, please ensure the presence of dry ice and transfer the vials immediately to -80°C storage.

Handling Notes: Avoid repeated freeze-thaw cycles. Aliquot into low-protein-binding tubes immediately after receipt to maintain optimal activity. For safety and contamination control, all viral handling must be performed inside a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: We ensure product integrity through rigorous QC, but we do not guarantee results in specific research contexts. Proper storage, handling, and total compliance with biosafety laws and safety protocols remain the sole responsibility of the user.

Target Profile

Gene Name: IFT27

Full Name: Intraflagellar transport 27

Gene Symbol: RAYL; BBS19; RABL4; FAP156; CFAP156

Gene ID: 11020

RefSeq ID-1: NP_001171172.1

RefSeq ID-2: NM_001177701.3

Summary: IFT27 encodes a GTP-binding protein that serves as a central component of the intraflagellar transport complex B. Studies of the homologous protein in Chlamydomonas suggest a role in cell cycle regulation. This gene undergoes alternative splicing, producing multiple transcript variants.

LipoKnoxa™ Human IFT27 shRNA Lentiviral Particle (Silencing)

Product Overview

Specification

Target Profile

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