Product Overview
Description:
AdipoUpX™ Human PPARG Lentiviral Particle (Overexpression) is a ready-to-use, high-titer vector particle. It is professionally engineered for the specific, efficient, and long-lasting stable overexpression of the human PPARG gene. The lentivirus characteristic of genome integration ensures the target gene integrates into the host cell genome, establishing reliable, stable cell lines for studying adipocyte differentiation, insulin sensitization, and lipid metabolism. Our vector system features a modular design, offering high flexibility for customization. Comprehensive quality control is performed to ensure both product safety and efficacy.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.
Sterility Test:
This product was certified sterile following successful microbial growth analysis.
Mycoplasma Test:
The production quality control confirmed the absence of any mycoplasmal agents.
Other QC:
This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.
Storage:
To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.
Stability:
Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.
Shipping Condition:
Our lentivirus viral products are shipped using dry ice.
Handling Notes:
Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.
Target Profile
Gene Name:
PPARG
Full Name:
Peroxisome proliferator activated receptor gamma
Gene Symbol:
GLM1; CIMT1; FPLD3; NR1C3; PPARG1; PPARG2; PPARG5; PPARgamma
Summary:
The PPARG gene is a critical regulator coding for peroxisome proliferator-activated receptor gamma, a central member of the nuclear receptor family. Its mechanism of action requires heterodimerization with retinoid X receptors (RXR), enabling it to function as a master transcription factor controlling pathways essential for adipogenesis (fat cell creation). As a molecular switch for lipid storage and glucose handling, dysregulation of PPAR gamma is heavily implicated in the pathogenesis of metabolic syndrome, including type 2 diabetes, severe obesity, atherosclerosis, and several malignancies. Multiple isoforms exist due to alternative splicing.
