AdipoUpX™ Human HSD11B1 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human HSD11B1 Lentiviral Particle (Overexpression) is professionally engineered for the long-lasting, stable expression of the human HSD11B1 gene. This enzyme converts inactive cortisone to active cortisol within adipose tissue, making this stable integration tool ideal for studying "local Cushing's syndrome" in obesity. The product is ready-to-use and high-titer, with every lot passing rigorous quality control for titer and purity. Clients can flexibly select reporter genes for precise tracking of glucocorticoid signaling effects.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: HSD11B1

Full Name: Hydroxysteroid 11-beta dehydrogenase 1

Gene Symbol: HDL; 11-DH; HSD11; HSD11B; HSD11L; CORTRD2; SDR26C1; 11-beta-HSD1

Gene ID: 3290

RefSeq ID-1: NP_001193670.1

RefSeq ID-2: NM_001206741.2

Summary: This gene encodes a microsomal enzyme that catalyzes the interconversion of cortisol and cortisone. Overactivity can lead to excess cortisol, contributing to central obesity. Variants in HSD11B1 have been linked to obesity and insulin resistance in children. Mutations in HSD11B1 and H6PD cause cortisone reductase deficiency. Alternate splicing produces multiple transcript variants encoding the same protein.

AdipoUpX™ Human HSD11B1 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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