AdipoUpX™ Human NR3C1 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human NR3C1 Lentiviral Particle (Overexpression) is a ready-to-use, high-titer vector particle professionally engineered for the stable overexpression of the human NR3C1 gene. As the primary receptor for glucocorticoids, it is a vital tool for researching how stress hormones influence adiposity and metabolic syndrome. The lentivirus characteristics ensure genome integration, establishing reliable stable cell lines. Every batch undergoes rigorous quality control, including titer, sterility, and mycoplasma testing, ensuring experimental reproducibility and safety.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: NR3C1

Full Name: Nuclear receptor subfamily 3 group C member 1

Gene Symbol: GR; GCR; GRL; GCCR; GCRST

Gene ID: 2908

RefSeq ID-1: NP_000167.1

RefSeq ID-2: NM_000176.3

Summary: NR3C1 encodes the glucocorticoid receptor, which acts both as a transcription factor binding glucocorticoid response elements and as a regulator of other transcription factors. The receptor is mainly cytoplasmic but translocates to the nucleus upon ligand binding, influencing inflammation, cell proliferation, and differentiation. Alternative splicing produces multiple isoforms with distinct nuclear-cytoplasmic dynamics. Dysregulation of NR3C1 may affect energy metabolism and contribute to obesity.

AdipoUpX™ Human NR3C1 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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