AdipoUpX™ Human PPARGC1A Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PPARGC1A Lentiviral Particle (Overexpression) is a powerful gene delivery tool specifically designed for the stable overexpression of the human PPARGC1A (PGC-1α) gene. This master regulator of mitochondrial biogenesis is essential for researching adaptive thermogenesis and metabolic flexibility. Genome integration enables the creation of stable cell lines for long-term functional validation. All particles undergo rigorous internal quality control validation to ensure reliability, and the vector system is highly customizable to meet diverse research needs.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: PPARGC1A

Full Name: PPARG coactivator 1 alpha

Gene Symbol: LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha)

Gene ID: 10891

RefSeq ID-1: NP_001317680.1

RefSeq ID-2: NM_001330751.2

Summary: PPARGC1A encodes a transcriptional coactivator that regulates genes involved in energy metabolism and mitochondrial function. It interacts with PPARγ and other transcription factors such as CREB and nuclear respiratory factors (NRFs), linking environmental cues to metabolic gene regulation. PPARGC1A plays a pivotal role in determining muscle fiber type, controlling cholesterol balance, and regulating blood pressure. Dysregulation of this gene has been implicated in obesity and other metabolic disorders.

AdipoUpX™ Human PPARGC1A Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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