AdipoUpX™ Human FTO Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human FTO Lentiviral Particle (Overexpression) is a powerful, high-titer gene delivery tool. It is specifically designed for the stable overexpression of the human FTO gene. The FTO protein is a key target for studying nucleic acid demethylation and fat accumulation regulation. Genome integration enables the establishment of stable cell lines. This product accelerates progress in obesity genetics by enabling rapid validation of FTO's effects on energy expenditure and metabolic signaling pathways. Customization of functional components, including promoters and markers, is highly flexible.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: FTO

Full Name: Alpha-ketoglutarate-dependent dioxygenase

Gene Symbol: GDFD; IFEX9; ALKBH9; BMIQ14

Gene ID: 79068

RefSeq ID: NM_001080432.3, NP_001073901.1

Summary: The FTO gene encodes a nuclear protein dioxygenase belonging to the AlkB family. Its core biochemical function involves participating in the oxidative demethylation of nucleic acids (DNA/RNA), considered a DNA repair mechanism. Functional studies have established FTO as one of the most significant genetic factors influencing BMI and obesity risk. Extensive data indicate that FTO functional defects are not only associated with type 2 diabetes but also play a substantial role in the nervous and cardiovascular systems, making it a focal point in research on genetic susceptibility to metabolic diseases.

AdipoUpX™ Human FTO Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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