AdipoUpX™ Human PHB1 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PHB1 Lentiviral Particle (Overexpression) is professionally engineered for the efficient and long-lasting overexpression of the human PHB1 (Prohibitin) gene. This mitochondrial protein is a reliable asset for studying mitochondrial health, cristae organization, and the prevention of obesity-induced oxidative damage. Every viral lot undergoes comprehensive quality control (titer, sterility, and mycoplasma detection) to ensure safety and efficacy. Clients can freely customize key functional components, including cell-specific promoters, to match their unique research environment.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: PHB1

Full Name: Prohibitin 1

Gene Symbol: PHB; BAP32; HEL-215; HEL-S-54e

Gene ID: 5245

RefSeq ID-1: NP_001268425.1

RefSeq ID-2: NM_001281496.2

Summary: PHB1 is an evolutionarily conserved gene implicated in cellular senescence, mitochondrial function, and tumor suppression. Its antiproliferative activity has been attributed to regulatory elements within the 3′ untranslated region, which may function as trans-acting RNA. PHB1 also plays roles in mitochondrial integrity and energy metabolism, linking it to obesity through regulation of adipocyte function and systemic metabolic homeostasis. Several pseudogenes and alternatively spliced transcript variants have been identified.

AdipoUpX™ Human PHB1 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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