AdipoUpX™ Human LCAT Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human LCAT Lentiviral Particle (Overexpression) is professionally engineered for the efficient and long-lasting overexpression of the human LCAT gene. This enzyme is critical for the maturation of HDL particles and reverse cholesterol transport; this stable integration system is an ideal tool for lipid metabolism research. Genome integration facilitates the establishment of permanent cell lines for long-term functional studies. Every batch is verified through comprehensive quality control screening for titer and purity, ensuring that experimental results remain safe and repeatable.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: LCAT

Full Name: Lecithin-cholesterol acyltransferase

Gene ID: 3931

RefSeq ID-1: NP_000220.1

RefSeq ID-2: NM_000229.2

Summary: LCAT is a blood-borne enzyme responsible for esterifying free cholesterol on HDL particles. This conversion is a prerequisite for effective reverse cholesterol transport, allowing cholesterol to be safely carried from peripheral tissues back to the liver. Genetic mutations in LCAT result in systemic cholesterol imbalances and "fish-eye disease," emphasizing the gene's importance in managing the cardiovascular risks associated with high BMI.

AdipoUpX™ Human LCAT Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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