AdipoUpX™ Human NTRK2 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human NTRK2 Lentiviral Particle (Overexpression) is a high-titer system for the specific and stable expression of the human NTRK2 (TrkB) gene. As the primary receptor for BDNF, its stable overexpression is essential for investigating satiety signaling and the prevention of hyperphagia. The lentivirus characteristic of genome integration ensures the establishment of reliable cell models for long-term functional validation. Comprehensive quality control is performed to ensure both product safety and the reproducibility of metabolic data.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: NTRK2

Full Name: Neurotrophic receptor tyrosine kinase 2

Gene Symbol: OBHD; TRKB; DEE58; trk-B; EIEE58; GP145-TrkB

Gene ID: 4915

RefSeq ID-1: NP_001007098.1

RefSeq ID-2: NM_001007097.3

Summary: NTRK2 codes for a membrane-bound kinase belonging to the neurotrophic tyrosine receptor kinase (NTRK) family, also known as TrkB. This receptor is activated upon binding to its neurotrophin ligands, leading to its autophosphorylation and the initiation of downstream cascades, notably the MAPK pathway. Signaling through NTRK2 is critical for neuronal cell differentiation and survival. Gene mutations or variants have been clinically associated with both obesity susceptibility and various mood disorders, suggesting a critical role in central nervous system energy balance and affective regulation.

AdipoUpX™ Human NTRK2 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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