AdipoUpX™ Human CASP1 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human CASP1 Lentiviral Particle (Overexpression) is a ready-to-use, high-titer vector particle designed for the stable overexpression of the human CASP1 gene. As the core of the inflammasome, its stable expression allows for systematic investigation into the maturation of IL-1B and its role in metabolic endotoxemia. To ensure the repeatability of your data, every batch undergoes rigorous quality control, including titer and sterility testing. Genome integration allows for the creation of reliable, stable cell lines for high-fidelity functional validation.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: CASP1

Full Name: Caspase 1

Gene Symbol: ICE; P45; IL1BC

Gene ID: 834

RefSeq ID-1: NP_001214.1

RefSeq ID-2: NM_001223.5

Summary: CASP1 encodes caspase-1, a cysteine-aspartic acid protease involved in both apoptosis and inflammation. It is best known for processing inactive pro–interleukin-1β into its active form, thereby promoting inflammatory responses. In obesity, CASP1-mediated inflammasome activation in adipose tissue contributes to chronic low-grade inflammation and insulin resistance. The gene produces multiple isoforms through alternative splicing.

AdipoUpX™ Human CASP1 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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