AdipoUpX™ Human CEBPA Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human CEBPA Lentiviral Particle (Overexpression) is a powerful, high-titer gene delivery tool specifically designed for the long-lasting overexpression of the human CEBPA gene. This transcription factor is a master regulator of terminal adipocyte differentiation; genome integration enables the establishment of stable cell lines to study the expansion of adipose tissue. All viral particle products undergo comprehensive quality control to ensure both safety and efficacy. Our modular design provides high flexibility for customization, allowing clients to choose from various cell-specific promoters.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: CEBPA

Full Name: CCAAT enhancer binding protein alpha

Gene Symbol: CEBP; C/EBP-alpha

Gene ID: 1050

RefSeq ID-1: NP_001272758.1

RefSeq ID-2: NM_001285829.2

Summary: CEBPA encodes a transcription factor with a basic leucine zipper (bZIP) domain that binds CCAAT motifs in target gene promoters. It forms homo- and heterodimers with CEBP family members, regulating genes involved in cell cycle control, adipocyte differentiation, and energy homeostasis. Disruptions in CEBPA activity may impact body weight regulation. Isoforms are generated from alternative start codons.

AdipoUpX™ Human CEBPA Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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