AdipoUpX™ Human RAI1 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human RAI1 Lentiviral Particle (Overexpression) is professionally engineered for the efficient and long-lasting overexpression of the human RAI1 gene. This transcription factor is critical for investigating Smith-Magenis syndrome and its associated obesity and circadian rhythm disruptions. Every batch is verified through comprehensive quality control screening (titer, sterility, and mycoplasma), ensuring that experimental results are safe and high-fidelity. Our vector system offers high flexibility for the selection of reporter genes for precise longitudinal validation.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: RAI1

Full Name: Retinoic acid induced 1

Gene Symbol: SMS; SMCR

Gene ID: 10743

RefSeq ID-1: NP_109590.3

RefSeq ID-2: NM_030665.3

Summary: RAI1 is located within the Smith–Magenis syndrome region on chromosome 17 and is highly expressed in neuronal tissues. The protein contains a polymorphic polyglutamine tract and is involved in transcriptional regulation. Altered RAI1 dosage is associated with hyperphagia, abnormal circadian rhythm, and syndromic obesity. In mouse models, neuronal expression of RAI1 is regulated by retinoic acid, supporting its role in neurodevelopmental and metabolic pathways.

AdipoUpX™ Human RAI1 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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