AdipoUpX™ Human PDE3B Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PDE3B Lentiviral Particle (Overexpression) is a high-titer system for the specific and stable expression of the human PDE3B gene. As a key enzyme in the antilipolytic action of insulin, its stable overexpression facilitates research into cAMP degradation and the regulation of fatty acid release from adipocytes. The lentivirus characteristic of genome integration establishes reliable cell models for signaling studies. Comprehensive quality control is performed on all particles to ensure safety, efficacy, and the highest standards for metabolic research.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: PDE3B

Full Name: Phosphodiesterase 3B

Gene Symbol: HcGIP1; cGIPDE1

Gene ID: 5140

RefSeq ID-1: NP_000913.2

RefSeq ID-2: NM_000922.3

Summary: PDE3B encodes a cyclic AMP phosphodiesterase that hydrolyzes cAMP and thereby modulates intracellular signaling pathways. It negatively regulates lipid catabolism and angiogenesis and participates in PI3K–AKT signal transduction. PDE3B is localized to membranes and functions within guanyl nucleotide exchange factor complexes. In adipocytes, PDE3B is a critical regulator of insulin-mediated suppression of lipolysis, making it highly relevant to obesity and insulin resistance.

AdipoUpX™ Human PDE3B Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

Related Products