AdipoUpX™ Human PNLIP Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PNLIP Lentiviral Particle (Overexpression) is a ready-to-use, high-titer vector particle designed for the stable overexpression of the human PNLIP gene. As the primary lipase for dietary fat digestion, its stable expression in cell models allows for in-depth research into fat absorption mechanisms. To ensure experimental reproducibility, the product undergoes comprehensive quality control, including titer and mycoplasma detection. Researchers can flexibly choose from various markers to track the expression and secretion of this crucial enzyme.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: PNLIP

Full Name: Pancreatic lipase

Gene Symbol: PL; PTL; PNLIPD

Gene ID: 5406

RefSeq ID-1: NP_000927.1

RefSeq ID-2: NM_000936.4

Summary: PNLIP encodes a pancreatic lipase enzyme that is secreted into the small intestine, where it catalyzes the breakdown of dietary triglycerides. This enzyme is essential for the efficient digestion and absorption of fats. Inhibition of PNLIP activity has been shown to reduce obesity induced by high-fat diets in mice and can promote weight loss in humans with obesity. Mutations in this gene cause congenital pancreatic lipase deficiency, a rare disorder characterized by fat malabsorption and steatorrhea.

AdipoUpX™ Human PNLIP Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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