AdipoUpX™ Human BBS4 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human BBS4 Lentiviral Particle (Overexpression) is professionally engineered for the efficient and long-lasting overexpression of the human BBS4 gene. This tool allows for the stable establishment of cell lines to study the recruitment of ciliary cargo and its impact on satiety signaling. Every batch is verified through comprehensive quality control screening for titer, sterility, and mycoplasma, ensuring the repeatability and safety of your experiments. Our vector system offers high flexibility for the selection of reporter genes and promoters.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: BBS4

Full Name: Bardet-Biedl syndrome 4

Gene ID: 585

RefSeq ID-1: NP_001239607.1

RefSeq ID-2: NM_001252678.2

Summary: BBS4 is a BBS gene family member involved in Bardet-Biedl syndrome, characterized by obesity, retinal dystrophy, polydactyly, renal anomalies, and cognitive deficits. BBS4 contributes to cilia formation and function, localizing to basal bodies, ciliary axonemes, and pericentriolar regions, and participates in microtubule-dependent intracellular trafficking. It also interacts with O-linked N-acetylglucosamine (O-GlcNAc) transferases to form the multi-protein BBSome complex. Multiple transcript variants are generated through alternative splicing.

AdipoUpX™ Human BBS4 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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